Parental functions during conjugation in Escherichia coli K-12.

INTRoDucTioN................................................................ 320 EXPERIMENTAL APPROACH...................................................... 321 MATERIALS AND METHODS..................................................... 321 Media................................................................... 321 Bacterial Strains............................................................ 322 Mating Procedures.......................................................... 322 Controls ................................................................... 322 Reproducibility of Experiments................................................ 324 RESULTS........................... 324 Effects of Inhibiting Energy Metabolism in One or Both Parents Before and During Conjugation .............................................................. 324 Effects of growth conditions and starvation.................................... 324 Effects of starvation for an energy source prior to and during mating.............. 327 Function of energy metabolism in each parent during conjugation................. 327 Effects of Inhibiting DNA Synthesis Before and During Conjugation................ 330 Recombinant production in matings with thymine-requiring parents.............. 330 DNA synthesis and initiation of chromosome transfer........................... 330 Effects of Inhibiting Protein Synthesis Before and During Conjugation ...... ......... 331 Effects of amino acid starvation on recombinant production...................... 331 Effects of completion of vegetative chromosome replication on recombinant production ................................................................... 331 Effects of Genetic Inhomology Between Donor and Recipient Chromosomes on Chromosome Transfer........................................................ 336 Effects of deletions in the Fchromosome on recombinant production by Hfr donors.. 337 Effects of deletions in the Hfr chromosome on recombinant production............. 340 Effects of deletions in the Fchromosome on F' transfer........................ 341 SUMMARY DISCUSSION......................................................... 344 LITERATURE CITED............................................................ 346